Description
This Eagle Biosciences ELISA Assay kit is for the determination of human PYY in samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to human PYY and biotin-avidin affinity system. To the wells of plate coated with rabbit anti human PYY antibody, standard or samples, labeled Antigens are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled streptoavidin-biotinylated Antigens-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3’,5,5’-Tetramethyl- benzidine (TMB) and the concentration of human PYY is calculated